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题名 白藜芦醇对过氧化氢损伤人肝癌细胞SMMC-7721的保护作用
姓名 张素英
院系 生命科学学院
专业 动物学
学位名称 理学硕士
外文题名 Prtective effect of resveratrol against injury induced by hydrogen peroxide in human hepatoma cells
第一导师姓名 常城
关键词 白藜芦醇;人肝癌细胞;过氧化氢;抗氧化酶;丙二醛
外文关键词 Resveratrol;Human hepatoma cell line SMMC-7721;Hydrogen peroxide;Antioxidant enzymes;Malondialdehyde
学科 理学
摘要 为了研究白藜芦醇对氧化损伤人肝癌细胞的保护作用,本实验采用过氧化氢诱导SMMC-7721 细胞进入氧应激状态。保护细胞采用两种方式:(1)白藜芦醇预先保护细胞1 h 后再加入H2O2 共同作用24 h;(2)先用H2O2 处理细胞,1 h后再加入白藜芦醇共同作用24 h。主要研究内容和结论如下: 1.体外人肝癌细胞氧化损伤模型的建立。不同浓度H2O2 对SMMC-7721 细胞的直接作用实验结果表明,低浓度(5-25 μM)过氧化氢对细胞MDA 含量没有影响;而较高浓度(50-200 μM)过氧化氢剂量依赖性地增加了细胞MDA 含量。低浓度(5 μM)过氧化氢对细胞活力没有影响,而较高浓度(25-200 μM)过氧化氢显著降低了细胞活力并呈剂量效应。本实验以100 μM H2O2 作用于SMMC-7721 细胞24 h 作为氧化损伤模型。 2.白藜芦醇对氧化损伤人肝癌细胞保护作用的研究。(1)白藜芦醇对SMMC-7721 细胞的直接作用实验结果表明:低浓度(10-50 μM)白藜芦醇对细胞活力及MDA 含量均无影响;高浓度(100-200 μM)白藜芦醇使细胞活力显著降低、MDA 含量显著升高。低浓度(10 μM)白藜芦醇对细胞SOD、CAT 和GPx活性均无影响,而较高浓度(25-200 μM)白藜芦醇使细胞SOD、CAT 和GPx 活性均呈剂量依赖性增加。(2)白藜芦醇预处理对过氧化氢损伤SMMC-7721 细胞的保护作用实验结果表明:低浓度(10-25 μM)白藜芦醇显著抑制了过氧化氢引起的SMMC-7721 细胞MDA 含量增加及细胞凋亡或坏死,而高浓度(100-200 μM)白藜芦醇显著促进了过氧化氢引起的SMMC-7721 细胞MDA 含量增加及细胞凋亡或坏死。白藜芦醇显著削弱了过氧化氢引起的SMMC-7721 细胞SOD、CAT 和GPx活性降低,并呈剂量依赖性。(3)过氧化氢损伤后白藜芦醇对SMMC-7721 细胞的保护作用实验结果表明:白藜芦醇对于过氧化氢引起的细胞活力下降、MDA含量升高及抗氧化酶(SOD、CAT 和GPx)活性下降均无影响。 结果表明,在过氧化氢损伤之前以低浓度(10-25 μM)白藜芦醇预处理,能有效地保护SMMC-7721 细胞免受氧化性损伤。可见,白藜芦醇的处理时间和 剂量决定了其对过氧化氢损伤SMMC-7721 细胞的作用。
外文摘要 The protective effect of resveratrol on human hepatoma cells (SMMC-7721) was investigated when cells were treated with reactive oxygen species in vitro. Hydrogen peroxide introduced SMMC-7721 cells into oxidative stress. Two means were used to protective SMMC-7721 cells: (1) cells were incubated with various concentrations of resveratrol for 1 h, followed by culture media containing both resvera¬trol and 100 μM hydrogen peroxide for another 24 h; (2) cells were incubated with 100 μM hydrogen peroxide for 1 h, followed by culture media containing both 100 μM hydrogen peroxide and res¬veratrol for another 24 h. The research contents and results were listed as follows: 1. The oxidative injury model of SMMC-7721 cells in vitro. Low concentrations (5-25 μM) of hydrogen peroxide had no effect on intracellular MDA content, while higher concentrations (50-200 μM) of hydrogen peroxide resulted in a dose-dependent increase of intracellular MDA content. Low concentration (5 μM) of hydrogen peroxide had no effect on cell viability, while higher concentr¬ations (25-200 μM) of hydrogen peroxide resulted in a dose-dependent reduction of cell viability. SMMC-7721 cells treated with 100 μM hydrogen peroxide for 24 h were regarded as oxida¬tive injury model in the study. 2. Protective effect of resveratrol against injury induced by hydrogen peroxide in SMMC-7721 cells. (1) Experiment of resveratrol incubation with normal SMMC-7721 cells showed low concentration (10 μM) of resveratrol had no effect on cell viability and intracellular MDA content, while high concentrations (100-200 μM) of resveratrol significantly reduced cell viability and enhanced intracellular MDA content. Low concentration (10 μM) of resveratrol had no effect on the activities of SOD, CAT and GPx, while higher concentrations (25-200 μM) of resveratrol significa¬ntly resulted in a dose-dependent increase of the activities of SOD, CAT and GPx. (2) When cells were pretreated with low concentration (10-25 μM) of resveratrol prior to challenge with 100 μM hydrogen peroxide, the increase induced by hydrogen peroxide in MDA content and the apoptosis or necrosis were significantly attenuated. In contrast, pretreatment with high concentrations (100-200 μM) of resveratrol enhanced hydrogen peroxide-induced increase in MDA content and the apoptosis or necrosis. Resveratrol significantly attenuated hydrogen peroxide-induced reduction in the activities of SOD, CAT and ...
研究领域 发育生物学
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